Fig. 3From: Metformin alleviates adriamycin resistance of osteosarcoma by declining YY1 to inhibit MDR1 transcriptional activityMet suppressed the expression of YY1 and MDR1 in MG-63/ADM cells. The mRNA expression levels of YY1 (A) and MDR1 (B) after Met treatment were detected via qRT-PCR. (C) The protein expression levels of YY1, P-gp, Bcl-2 and Bax following Met exposure were detected by Western blotting. (D) Quantitative analysis of Western blot and GAPDH as internal reference. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *P < 0.05, ***P < 0.001Back to article page