Fig. 4

YY1 promoted MDR1 expression by directly binding to its promoter (A) The expression of YY1 after transfection with YY1 overexpression vector was measured via qRT-PCR. (B) The protein expression of YY1 after transfection with YY1 overexpression vector was detected by Western blotting. (C) The mRNA levels of MDR1 were measured via qRT-PCR. (D) P-gp protein expressions were detected by western blotting. (E) Dual-luciferase reporter detection was carried out to measure the transcriptional activity of MDR1 with YY1 overexpression. (F) The targeting relationship between YY1 and MDR1 was determined by ChIP-qPCR assay. (G) Agarose gel electrophoresis of ChIP-qPCR results were assayed. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001