Fig. 1

Calycosin reduced inflammation in ovalbumin (OVA)-induced asthmatic mice. (A) Total inflammatory cells, including (B) eosinophils, (C) neutrophils, (D) macrophages and (E) lymphocytes, were counted in the bronchoalveolar lavage fluid (BALF). (F) ELISA assay evaluated the levels of serum immunoglobulin E (IgE). (G) The lung sections were stained with Hematoxylin-Eosin (H&E). The degree of inflammatory cell infiltration was scored (0–4). (H) The lung sections were stained with Periodic acid-Schiff (PAS). The degree of airway wall thickening and mucus hypersecretion was scored (0–4). Scale bar = 100 μm. n = 6 for each group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with the sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with the OVA group. “Ca” represents “calycosin”