Microvascular endothelial cells were isolated from hearts of wild-type (WT) and ATGL(−/−) mice and immortalized to create WT-MyEnd and ATGL(−/−)-MyEnd cells, respectively. Cells were cultured to passage 2–4 and characterized for different parameters of PPARα and NO signaling. Real-time PCR analysis revealed that PPARα mRNA expression was reduced more than 50% in ATGL(−/−)-MyEnd cells, which reflects well the situation in ATGL-deficient hearts. By contrast, mRNA expression of peroxisome proliferator-activated receptor-gamma coactivator (PGC-1α) was similar in WT and ATGL-deficient cells. Likewise, mRNA levels of different PPARα target genes were unaffected by knockout of ATGL. Protein expression and activity of endothelial nitric oxide synthase (eNOS) were measured by Western blot and conversion of L-[3H]arginine into L-[3H]citrulline, respectively. Both parameters were almost identical in WT-MyEnd and ATGL(−/−)-MyEnd cells. To investigate if accelerated breakdown of NO due to increased formation of reactive oxygen species (ROS) occurs in ATGL-deficient endothelial cells, we measured mRNA and protein expression of xanthine oxidase (XO) and NADPH oxidase isoforms NOX2 and NOX4. However, no differences in mRNA or protein expression were observed. A similar result was achieved in experiments measuring ROS formating in homogenates of WT and ATGL-deficient cells using lucigenin-enhanced chemiluminescence.