Neurobiological correlates of successful deep brain stimulation in a mouse model of high trait affect

Recent evidence suggests that high-frequency deep brain stimulation of the nucleus accumbens (NAcb-DBS) may represent a novel therapeutic strategy for individuals suffering from treatment-resistant depression although the underlying mechanism of action remains largely unknown. Using a unique psychopathological mouse model of enhanced depression- and anxiety-like behavior (HAB) we investigated behavioral and neurobiological effects of NAcb-DBS.

HAB mice underwent either chronic treatment with different selective serotonin reuptake inhibitors (SSRIs) or stereotactic surgery to implant DBS electrodes into the NAcb. NAcb-DBS was applied for 1 h daily for seven consecutive days (130 Hz, 100 µA, 60 µs pulse width) and sham-stimulated animals were used as controls. Anxiety- and depression-related behaviors were assessed using established tests with predictive anxiolytic or antidepressant validity. Furthermore, the effects of NAcb-DBS on challenge-induced immediate early gene expression and hippocampal neurogenesis were investigated.

Chronic SSRI treatment did not alter the enhanced depression-like behavior of HAB mice, while repeated, but not single, NAcb-DBS induced robust antidepressant and anxiolytic responses. Interestingly, NAcb-DBS did not affect behavior in normal depression/anxiety animals (NAB), suggesting a preferential effect of NAcb-DBS on pathophysiologically deranged systems. Antidepressant-like effects of NAcb-DBS were associated with normalization of challenge-induced dentate gyrus hypoactivity and modulation of neuronal activity in various brain regions implicated in stress and depression. Furthermore, NAcb-DBS enhanced the blunted hippocampal neurogenesis in HABs.

Taken together we show that the normalization of pathophysiologically enhanced depression-like behavior by repeated NAcb-DBS was associated with normalization of aberrant brain activity and rescue of impaired adult neurogenesis, indicating that DBS affects gene expression as well as neuronal plasticity in a defined, mood-associated network. Finally, it is suggested that SSRI-insensitive HAB mice represent a clinically relevant model for elucidating the neurobiological correlates underlying the observed behavioral effects of NAcb-DBS.

Supported by the Hope for Depression Research Foundation (HDRF/ISAN) and the Austrian Science Fund FWF DK SPIN (W1206).

Affiliations

1. Department of Pharmacology and Toxicology, Leopold-Franzens-University of Innsbruck, 6020, Innsbruck, Austria

   • Claudia Schmuckermair
   • , Stefano Gaburro
   • , Anupam Sah
   • , Simone B Sartori
   •  & Nicolas Singewald

2. Department of Physiology, Westfälische Wilhelms-University Münster, 48149, Münster, Germany

   • Stefano Gaburro

3. Max Planck Institute of Psychiatry, 80804, Munich, Germany

   • Rainer Landgraf

Corresponding author

Correspondence to Nicolas Singewald.

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