Skip to main content

Advertisement

Volume 13 Supplement 1

18th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

The differential effect of resveratrol on the renal artery of normal and diabetic rats

BMC Pharmacology and Toxicologyvolume 13, Article number: A48 (2012) | Download Citation

Article metrics

  • 1060 Accesses

  • 2 Citations

Background

Resveratrol, a polyphenol present in red wine, is thought to be responsible for cardiovascular benefits associated with moderate wine consumption. The mechanisms by which resveratrol causes vasodilatation are uncertain. The aim of this study was to investigate the mechanisms of resveratrol-induced vasorelaxation of rat renal artery (RA) with endothelium in normal and diabetic rats.

Methods

Alloxan was used for the induction of diabetes in rats. Samples of RA were obtained from male Wistar rats and were mounted in an organ bath for recording isometric tension. The experiments followed a multiple curve design.

Results

Resveratrol relaxed RA of normal rats more potently than RA of rats with diabetes (EC50 8 and 50 µM, respectively). L-NAME and methylene blue partly antagonized the relaxation of RA of normal animals only. A nonselective blocker of voltage-gated K+ (KV) channels, 4-aminopyridine (4-AP) partly inhibited the relaxation of RA of normal as well as of diabetic rats. However, margatoxin, a selective antagonist of KV1.x channels, completely antagonized the relaxation of RA of diabetic rats only. Glibenclamide, a highly selective blocker of ATP-sensitive K+ channels, did not block resveratrol-induced relaxation in both experimental models.

Conclusions

In conclusion, we have shown that resveratrol induces a strong endothelium-dependent relaxation of RA of normal rats, and that 4-AP-sensitive K+ channels are involved in this relaxation. In diabetic rats, resveratrol induced NO-independent relaxation and maragtoxin-sensitive K+ channels are involved.

Acknowledgements

This work has been supported by scientific research grants no. TP31020 from the Ministry of Science, Republic of Serbia.

Author information

Affiliations

  1. Institute of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of Belgrade, 11129, Belgrade, Serbia

    • Ljiljana C Gojković-Bukarica
    • , Radmila B Novaković
    •  & Dragana D Protić

  2. Serbian Academy of Sciences and Arts, 11129, Belgrade, Serbia

    • Vladimir I Kanjuh

  3. Department of Pharmacy, Faculty of Medicine, 21000, Novi Sad, Serbia

    • Jelena M Cvejić
    •  & Milica T Atanacković

Authors

  1. Search for Ljiljana C Gojković-Bukarica in:

  2. Search for Vladimir I Kanjuh in:

  3. Search for Radmila B Novaković in:

  4. Search for Dragana D Protić in:

  5. Search for Jelena M Cvejić in:

  6. Search for Milica T Atanacković in:

Corresponding author

Correspondence to Ljiljana C Gojković-Bukarica.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Keywords

  • Methylene Blue
  • Polyphenol
  • Renal Artery
  • Resveratrol
  • Glibenclamide

BMC Pharmacology and Toxicology

ISSN: 2050-6511

Contact us