- Meeting abstract
Does pulmonary surfactant generally affect antimicrobial activity?
BMC Pharmacology and Toxicologyvolume 13, Article number: A62 (2012)
Activity of antimicrobial agents may be affected by pulmonary surfactant. Notably, it was reported that the clinical efficacy of daptomycin is significantly impaired in pneumonia in spite of bacterial susceptibility in vitro. This study set out to assess the impact of pulmonary surfactant in vitro on bacterial killing of other antibiotics used for treatment of pneumonia.
Time-kill curves of daptomycin, doripenem, linezolid, moxifloxacin, and tigecycline were determined for Staphylococcus aureus ATCC 29213 and of colistin, doripenem and moxifloxacin for a clinical isolate of Pseudomonas aeruginosa at concentrations above or equal to the respective MICs. All experiments were performed over 24 h in Mueller-Hinton broth (MHB) and in MHB enriched with porcine surfactant at a concentration of 1 mg/mL (MHBsurf).
As expected, daptomycin was not bactericidal in presence of surfactant at concentrations up to 64 times the MIC. In MHBsurf a higher concentration of moxifloxacin (16x) was needed than in MHB (2x MIC) to achieve sustained bacterial killing of S. aureus. In contrast, killing of P. aeruginosa by moxifloxacin was not affected by surfactant. A slightly higher concentration of doripenem (8x) was needed in MHBsurf to achieve sustained antimicrobial killing against S. aureus than in MHB (4x MIC). However, killing was faster in MHBsurf. Similarly, initial killing of S. aureus by tigecycline was faster in MHBsurf than in MHB while after 24 hours no difference in bacterial counts was observed between MHB and MHBsurf. For linezolid no significant effects were observed by adding surfactant. Likewise, surfactant had no significant influence on the activity of colistin and doripenem against P. aeruginosa.
The activity of moxifloxacin against S. aureus was reduced in vitro by addition of surfactant whereas this effect could not be observed against P. aeruginosa. Interestingly, antimicrobial killing by several antibiotics of Gram-positive S. aureus, but not of Gram-negative P. aeruginosa tended to be faster in presence of surfactant. Thus, apart from daptomycin, pulmonary surfactant is also capable of influencing the bacterial killing kinetics of several other antibiotics. The clinical relevance of these in vitro findings for pneumonia patients is currently unclear, and should be carefully evaluated.