A TRPC3 blocker, Pyr3, prevents stent-induced arterial remodeling

Background

TRPC-mediated Ca²⁺ entry has been implicated in the control of smooth muscle proliferation and might represent a pivotal mechanism underlying in-stent restenosis. As we have observed significant expression of TRPC3 in human smooth muscle from coronary arteries as well as from aorta, we tested the efficiency of a recently discovered TRPC3-selective Ca²⁺ entry blocker, Pyr3 (10 µM) to prevent vascular smooth muscle proliferation and stent implantation-induced hyperplasia of human aorta.

Methods and results

The effect of Pyr3 on proliferation was measured by detection of BrDU incorporation and PCNA expression in human coronary smooth muscle and microvascular endothelium, which displays significantly smaller expression levels of TRPC as compared to smooth muscle. Pyr3 inhibited smooth muscle proliferation with an IC₅₀ of about 3 µM but lacked detectable effects on endothelial proliferation. Measurements of ATP-induced Ca²⁺ signals revealed that Pyr3 suppressed agonist-induced Ca²⁺ entry more effectively in vascular smooth muscle as compared to endothelial cells. Inhibitory effects of Pyr3 on stent implantation-induced arterial injury were tested using a novel in vitro model of in-stent hyperplasia in human arteries based on organ-typical culture of human aortic constructs. Pyr3 (10 µM) effectively prevented increases in tissue levels of proliferation markers (PCNA and Ki67) at 2 weeks after stent implantation into human aortae. Similarly, proliferation markers were significantly suppressed when implanting a Pyr3-releasing stent prototype as compared to a bare metal stent control.

Conclusions

Our results suggest TRPC3 as a potential target for pharmacological control of smooth muscle proliferation. Selective inhibition of TRPC Ca²⁺ entry channels in vascular smooth muscle is suggested as a promising strategy for in-stent restenosis prevention.