Figure 10

Cell cycle-specific MeC/DAPI codistribution patterns. Flow cytometry results show DU145 cell populations were efficiently arrested in G0/G1-phase (A) and enriched in G2-phase (B). The culture conditions were chosen to skip an enrichment of the cells in S-Phase. G1-cells and G2-cells from parallel populations were subjected to immunofluorescence and confocal imaging. The prototypic nuclei (C and D) of the two cell cycle phases (with a low KL-value) show very similar MeC (green) and DAPI/gDNA (blue) codistribution patterns, also confirmed by their respective scatter plots (E and F).