Figure 5

Suppression of eIF4F protects against chemotherapy-induced cell death in non-transformed BJ/hTERT cells. (A) Representative Giemsa staining of BJ/hTERT cells pre-treated with nutlin-3a or Cr131-b for 24 hrs, or transfected with siRNA against eIF4E (si4E) (2 days before treatment), followed by removal of compounds and exposure to paclitaxel (PAC) for 48 hrs. Cells were then allowed to recover for 5 days. (B) Western blot analysis of p53 and eIF4E from BJ/hTERT cells treated with nutlin-3a, Cr131-b or transfected with siRNA against eIF4E. (C) Representative cell cycle profiles of BJ/hTERT cells pre-treated as described in Panel A. (D) Quantification of the DNA content of BJ/hTERT cells pre-treated as indicated in Panel A. n = 3. Bars denote S.E.M. (E) Relative viability of BJ/hTERT cells that had been pre-treated with nutlin-3a, Cr131-b or hippuristanol (Hipp) for 24 hours or transfected with si4E or a non-targeting control (siNT) 2 days prior to the indicated drug treatments. Cell counts for VRL, NOCO, and PAC are normalized to controls in which cells were exposed to vehicle. n = 3. Bars denote S.E.M.