Expression of proteins involved in apoptosis. Panel A: Bax and Bcl-xL/S expression determined by Western Blot employing specific antibodies in MDA-MB-231 cells treated for 72 h with 25 μM Gli (Gli) or vehicle (control) cells. The figure shows a representative Western blot of three independent experiments and the quantification of bands obtained for Bax and Bcl-xL/S protein. Bars represent the mean ± SEM of three independent experiments. p: Non significant (NS), t test. Panel B: Expression of Bcl-2 and Bax protein in MDA-MB-231 cells treated by 72 h with 25 μM Gli (Gli) or vehicle, obtained by flow cytometry. Bars represent the mean fluorescence ± SEM obtained by three independent experiments. p: NS, t test.