Table 3 Mean values of estimated LD 50 of Yessotoxin in different pathways using 4-level RSP designs
From: The development of response surface pathway design to reduce animal numbers in toxicity studies
Pathway | Dose on design level 1 | Dose on design level 2 | Dose on design level 3 | Dose on design level 4 | Mean LD50 (μg/kgBW) | 95% CI of Mean LD50 (μg/kgBW) |
|---|---|---|---|---|---|---|
4-Level RSP design with binomial outcome, k-adjustment factor and 9 animals at each design level | ||||||
01 | 400 M = 3.29 | 581 M = 6.23 | 499 M = 5.01 | 462 M = 4.38 | 457 | 449 – 466 |
02 | 400 M = 3.29 | 581 M = 6.23 | 499 M = 5.01 | 536 M = 5.07 | 513 | 510 – 516 |
03 | 400 M = 3.29 | 219 M = 0 | 301 M = 1.05 | 338 M = 1.97 | 391 | 389 – 392 |
4-Level RSP design with binomial outcome, k-adjustment factor and increasing number of mice with increasing design levels | ||||||
01 | 400 M = 1.10 | 581 M = 3.35 | 499 M = 3.9 | 462 M = 4.41 | 464 | 449 – 479 |
02 | 400 M = 1.10 | 581 M = 3.35 | 499 M = 3.9 | 536 M = 5.57 | 510 | 501 – 520 |
03 | 400 M = 1.10 | 219 M = 0 | 301 M = 0.82 | 338 M = 1.96 | 379 | 374 – 384 |
4-Level RSP design with multinomial outcome, k-adjustment factor and increasing number of mice with increasing design levels | ||||||
01 | 400 M = 1.10 | 482 M = 2.45 | 499 M = 3.87 | 502 M = 5.06 | 501 | 498 – 503 |
02 | 400 M = 1.10 | 482 M = 2.45 | 499 M = 3.87 | 496 M = 4.97 | 490 | 470 – 510 |
03 | 400 M = 1.10 | 482 M = 2.45 | 465 M = 3.48 | 468 M = 4.51 | 470 | 432 – 508 |
04 | 400 M = 1.10 | 482 M = 2.45 | 465 M = 3.48 | 462 M = 4.41 | 455 | 353 – 557 |