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Table 3 Mean values of estimated LD 50 of Yessotoxin in different pathways using 4-level RSP designs

From: The development of response surface pathway design to reduce animal numbers in toxicity studies

Pathway Dose on design level 1 Dose on design level 2 Dose on design level 3 Dose on design level 4 Mean LD50 (μg/kgBW) 95% CI of Mean LD50 (μg/kgBW)
4-Level RSP design with binomial outcome, k-adjustment factor and 9 animals at each design level
01 400 M = 3.29 581 M = 6.23 499 M = 5.01 462 M = 4.38 457 449 – 466
02 400 M = 3.29 581 M = 6.23 499 M = 5.01 536 M = 5.07 513 510 – 516
03 400 M = 3.29 219 M = 0 301 M = 1.05 338 M = 1.97 391 389 – 392
4-Level RSP design with binomial outcome, k-adjustment factor and increasing number of mice with increasing design levels
01 400 M = 1.10 581 M = 3.35 499 M = 3.9 462 M = 4.41 464 449 – 479
02 400 M = 1.10 581 M = 3.35 499 M = 3.9 536 M = 5.57 510 501 – 520
03 400 M = 1.10 219 M = 0 301 M = 0.82 338 M = 1.96 379 374 – 384
4-Level RSP design with multinomial outcome, k-adjustment factor and increasing number of mice with increasing design levels
01 400 M = 1.10 482 M = 2.45 499 M = 3.87 502 M = 5.06 501 498 – 503
02 400 M = 1.10 482 M = 2.45 499 M = 3.87 496 M = 4.97 490 470 – 510
03 400 M = 1.10 482 M = 2.45 465 M = 3.48 468 M = 4.51 470 432 – 508
04 400 M = 1.10 482 M = 2.45 465 M = 3.48 462 M = 4.41 455 353 – 557
  1. The results are expressed with 95% Confidence interval. M represents the mean of dead mice based on 10,000 simulations on the assigned dose.