Figure 3

CNX-011-67 increases islet insulin secretion and content. Insulin secretion (A), islet insulin content (B), ratio of secreted insulin to insulin content represented as percentage (C) of islets from sham control, n-STZ and n-STZ-CNX-011-67 animals after treatment with CNX-011-67 for 8 weeks. Freshly isolated rat islets were preincubated with 2.8 mM glucose for 30 min and later incubated with 5.6 mM and 16.7 mM glucose for 120 min. Both insulin secretion and islet insulin content were measured. Secreted insulin and islet insulin content are presented as ng of insulin/mg protein. (D) CNX-011-67 increases insulin secretion in human islets from T2DM patients. The islets from T2DM did not show glucose responsiveness as elevated glucose concentration (8 mM) could not increase insulin secretion. CNX-011-67 treatment resulted in increased glucose responsiveness as CNX-011-67 facilitated insulin secretion only at higher glucose concentration. Values represent mean ± S.E.M (n = 4). Statistical analysis was performed by Students t-test (Two-way, unpaired: *P < 0.05, **P < 0.01 and ***P < 0.001).