Figure 4

CNX-011-67 improves islet insulin content, β-cell mass and number-immuno fluorescence staining and elctron microscope studies of CNX-011-67 treatment in islets and β-cell. Sections from sham control, n-STZ and n-STZ-CNX-011-67 Islets stained for insulin content using Immuno fluorescent staining (FITC; green) (A). Double immuno fluorescence staining for PDX1 (red; alexafluor 555) and insulin (green; FITC) representing all groups (B). Electron microscope images of β-cells from sham control, n-STZ and n-STZ-CNX-011-67 animals, treated animals showed improvement and increased number of dense-core insulin granules when compared with n-STZ animals. Mitochondria and endoplasmic reticulum health have restored in treatment (C). White arrow: nucleus, white arrowheads: mitochondria, black arrowheads: insulin vesicles. The n-STZ animals showed decreased β-cell number and mass when compared with normal control animals. The treated animals exhibited non significant increase in the β-cell number and β-cell mass (D & E). Images were taken at 40× magnification for immunohistochemical analysis and 4800× magnification for electron micrographs. Scale bar: 5 μm. Data in all panels are mean ± S.E.M (n = 6). Statistical analysis was performed by Students t-test (two-way, unpaired: *P < 0.05 and ***P < 0.001).