Representative agarose gel images assessing genotypes and recombination in mice. A PCR genotyping was done simultaneously using three primers A, B, and C from Figure 1. A 492-bp band represents the wild-type Gnai2 allele and the 745-bp band represents the Gnai2 fl-G184S conditional allele after removal of the neo cassette. B Genomic DNA extracted from blood was amplified before and after tamoxifen treatment. Lanes 1 and 2 were from Cre negative Gα fl-G184S/fl-G184S mice while lanes 3-6 were from Cre-ERT2, Gαi2
fl-G184S/fl-G184S mice. Tamoxifen-induced recombination was evaluated using primers A and B and confirms the in vivo conversion to the *G184S allele for expression of the RGS-insensitive Gαi2. The 659-bp band was exclusively generated after tamoxifen treatment (Tam). The non-recombined fl-G184S allele is not detected due to the large size of the PCR product (3189-bp).