Figure 4

Gα _i2 protein expression is reduced in mice conditionally expressing Gα _i2 ^G184S . Gα_i2 protein expression was measured by Western blot in hearts (A), brains (B), and kidneys (C) isolated from mice treated with tamoxifen (50 mg/kg; shaded bars) or vehicle (nonshaded bars) for 5 consecutive days. The membrane was stripped and reblotted with a GAPDH antibody as a loading control. Values represent the mean ± SE of tissues from three different animals for each genotype. Data are normalized to Gα_i2 protein expression in tissues from wildtype C57B/6J mice that do not express Cre and do not carry the Gα_i2 ^G184S mutation. Panel D shows a representative blot of kidneys from three different animals of each genotype. * and ** indicate significant differences (p < 0.05 and p < 0.01, respectively) relative to control mice (G_αi2 ^+/+) that do not carry the Cre-ER^T2 or G_αi2 ^{fl-G184S/fl-G184S} genes.