Figure 1

Schematic representation of a single RM20S® syringe combined with British American Tobacco’s exposure chamber (UK patent publication WO 03/100417/ A1) (not to scale). The RM20S® can smoke up to eight cigarettes simultaneously. [A] Cigarette holder with cigarette in place; [B] 150 mL glass syringe where cigarette smoke dilution in air is prepared; [C] Plunger; [D] Exposure chamber containing porous membrane inserts with cells seeded on top at the ALI [E] Transwell® insert representation. Figure adapted from [26].