Figure 1
Substrate profiles of (MOUSE)NAT2*1, (MOUSE)NAT2_F125S and (MOUSE)NAT1*1. Substrate specific activity profiles of (MOUSE)NAT2*1 (black columns), (MOUSE)NAT2_F125S (dashed white columns) and (MOUSE)NAT1*1 (gray columns) are shown. Maximal specific activity values (normalised as 100% relative specific activity) were: 196 μM.sec−1.mg−1 for (MOUSE)NAT2*1 with 4ABA, 25 μM.sec−1.mg−1 for (MOUSE)NAT2_F125S with ANS and 104 μM.sec−1.mg−1 for (MOUSE)NAT1*1 with INH. A dashed line separates aromatic amines from hydrazines. The circles above the bars show the significance of the differences between (MOUSE)NAT2_F125S or (MOUSE)NAT1*1 and (MOUSE)NAT2*1 (●) and between (MOUSE)NAT2_F125S and (MOUSE)NAT1*1 (○) using ANOVA with Student’s t-test; ● or ○: p ≤0.05; ●● or ○○: p ≤0.01; ●●● or ○○○: p ≤0.001.