Fig. 3

Cellular GSH content of ABCB5- and Abcb5-transfected cells. a–c HPLC chromatograms of AQC-derivatized methanol extracts of 293/mock (a), 293/B5-11 (b) and 293/mb5-8 (c) cells. GSH peaks indicated by arrows were detected at 20 min. d Cellular GSH content in 293/mock, 293/B5-11 and 293/mb5-8 cells determined by HPLC. The data are presented as mean ± SD of triplicate determinations. Asterisks show p < 0.05 by two-sided t-test. e, f HPLC chromatograms of AQC-derivatized methanol extracts of 293/mock (e) and 293/B5-11 (f) cells after incubation for 2 days in the presence of 100 μM BSO. g Intracellular accumulation of GSH in 293/mock and 293/B5-11 cells. Cells were incubated with 1 nM [³H]GSH at 37 °C for 0, 2, 5 and 10 min. h ATP-dependent uptake of GSH in 293/mock and 293/B5-11 vesicles. The membrane vesicles were incubated at 25 °C with 66 nM [³H]GSH in the absence or presence of 3 mM ATP for 0, 2 and 10 min