Fig. 5
From: Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells
Glutathione metabolism of ABCB5- and Abcb5-transfected cells. a mRNA expression of genes encoding enzymes involved in glutathione metabolism. PCR cycles were as follows: GCLC, 29, 32 and 35; GCLM, 27, 30 and 33; GSS, 29, 32 and 35; GSR, 27, 30 and 33; GSTP1, 27, 30 and 33; GAPDH, 20, 22 and 24. b Cellular amino acid contents in 293/B5-11 and 293/mb5-8 cells. The methanol extracts of cells were derivatized using 4-fluoro-7-nitro-2,1,3-benzoxadiazole and subjected to HPLC. Data represent the percentage relative to 293/mock cells for each amino acid. The data are presented as mean ± SD of triplicate determinations