Table 1 Sequences of primers used in RT-PCR analysis
From: Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells
Gene | Primer orientation | Sequence |
|---|---|---|
GCLC | forward | 5'-GTTCTCAAGTGGGGCGATGA-3' |
reverse | 5'-CCGGCTTAGAAGCCCTTGAA-3' | |
GCLM | forward | 5'-AAGTGCAGTTGACATGGCCT-3' |
reverse | 5'-TGACCGAATACCGCAGTAGC-3' | |
GSS | forward | 5'-GACCAGCGTGCCATAGAGAA-3' |
reverse | 5'-ACCCTCTCTCTGGGGCTTTA-3' | |
GSR | forward | 5'-GTGGCACTTGCGTGAATGTT-3' |
reverse | 5'-AGTTTTCGGCCAGCAGCTAT-3' | |
GSTP1 | forward | 5'-CAAGTTCCAGGACGGAGACC-3' |
reverse | 5'-GCCATTGATGGGGAGGTTCA-3' | |
GAPDH | forward | 5'-ATCACCATCTTCCAGGAGCGA-3' |
reverse | 5'-GCTTCACCACCTTCTTGATGT-3' |