Fig. 3

l-THP effect on nicotine-induced hyperactivity. l-THP displayed a protective effect against nicotine induced hyperactivity. In testing of hyperactivity, all rats were pretreated with two injections of saline during the baseline phase. In the nicotine administration phase (Nic Admin) rats were pretreated with their respective treatment before placement into chambers and treated with nicotine 5 min before placement into chambers. a Total movements displayed over each session. All groups display similar movements from acclimation through pretreatment test. When groups are given their respective pretreatments followed by nicotine, differences emerge between groups, significance is seen individually on day 13 (Nic 6) *p < 0.05. b Hyperactivity testing collapsed by segment: Baseline, Nic Admin, or Nic Challenge. During the nicotine administration phase rats pretreated with l-THP did not display hyperactivity compared to their respective baseline activity. Rats pretreated with saline did display hyperactivity compared to their baseline. During the nicotine challenge (Nic Challenge) all rats received only one injection of nicotine (0.4 mg/kg s.c.) 5 min before placement into locomotor chambers. All rats displayed hyperactivity in this phase. Rats previously pretreated with l-THP who did not display hyperactivity during the nicotine administration phase rebounded to display hyperactivity when l-THP was not presented before nicotine. All data is presented as mean + S.E.M. Two-way ANOVA * denotes within group comparison to baseline, **p < 0.01, ***p < 0.001, + denotes between group comparison of l-THP and saline during Nic Admin, student paired t-test, +++ p < 0.001, n = 8 per group