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Table 1 Vesicle, clustering, and mitochondrial measurements in Dentate Gyrus –Perforant Pathway (DG-PP) synapses and CA3-Mossy Fiber Pathway (CA3-MF) Synapses

From: Chronic early life lead (Pb2+) exposure alters presynaptic vesicle pools in hippocampal synapses

  CA3 – Mossy Fiber Terminals Dentate Gyrus – Perforant Pathway Terminals
  Control (Mean ± SEM) Pb2+ (Mean ± SEM) P value Control (Mean ± SEM) Pb2+ (Mean ± SEM) P value
I. Raw Vesicle Counts
 A) Rapidly Releasable/Docked Vesicle Pool 8.527 ± 0.8456 6.018 ± 0.001 0.0236 7.013 ± 1.003 5.232 ± 0.9928 0.0989
 B) Recycling Vesicle Pool 35.19 ± 7.804 29.26 ± 7.381 0.0187 31.48 ± 9.315 26.09 ± 5.835 0.1814
 C) Resting Vesicle Pool 43.64 ± 5.570 51.80 ± 7.427 0.2271 51.66 ± 8.374 59.80 ± 11.44 0.2586
 D) Total Number of Vesicles 87.36 ± 10.61 87.17 ± 1.761 0.4936 90.07 ± 9.363 91.12 ± 10.46 0.4603
II. Vesicular Diameters
 A) Rapidly Releasable/Docked Diameter 20.67 ± 1.644 21.20 ± 1.137 0.3995 20.82 ± 1.521 23.27 ± 2.12 0.3894
 B) Recycling Vesicle Diameter 22.09 ± 0.8383 22.77 ± 0.6942 0.2799 22.10 ± 0.8234 24.41 ± 2.072 0.1695
 C) Resting Vesicle Diameter 24.83 ± 1.746 24.70 ± 1.322 0.4764 24.69 ± 2.481 26.28 ± 2.506 0.3343
 D) Average Diameter of all vesicles 23.77 ± 1.406 23.84 ± 0.8775 0.4843 23.72 ± 1.565 25.64 ± 2.094 0.2449
III. Length Measurements
 A) PSD length 309.1 ± 13.48 259.7 ± 18.74 0.0382 269.3 ± 9.680 266.6 ± 15.31 0.4432
 B) PAZ length 311.6 ± 11.15 281.2 ± 19.53 0.0899 265.6 ± 14.45 308.2 ± 26.90 0.1553
IV. Nearest Neighbor Distance (Clustering)
 A) Rapidly Releasable/Docked + Recycling (0–200 nm) 40.19 ± 1.398 45.56 ± 1.498 0.0062 41.75 ± 0.7873 48.57 ± 1.115 0.0001
 B) Resting (201–500 nm) 42.04 ± 1.834 48.47 ± 1.157 0.0020 45.35 ± 0.7979 51.19 ± 0.7168 0.0001
V. Mitochondrial Measurements
 A) Terminals with mitochondria 15.40 ± 2.227 17.60 ± 1.806 0.2340 27.00 ± 3.050 22.60 ± 3.280 0.1793
 B) Terminals with multiple mitochondria 2.00 ± 0.5477 1.250 ± 0.2500 0.1316 4.200 ± 0.9695 2.600 ± 0.5099 0.0970
 C) Total number of mitochondria 48.60 ± 6.577 54.20 ± 7.690 0.5972 69.60 ± 0.4964 62.80 ± 0.2518 0.1375
 D) Diameter of mitochondria 231.6 ± 16.18 224.4 ± 15.53 0.7566 221.8 ± 0.8072 226.5 ± 8.529 0.3500
 E) Large mitochondria (300 nm or greater) 4.800 ± 1.594 6.800 ± 0.9695 0.1624 8.00 ± 2.345 7.200 ± 1.530 0.3924