Table 1 Vesicle, clustering, and mitochondrial measurements in Dentate Gyrus –Perforant Pathway (DG-PP) synapses and CA3-Mossy Fiber Pathway (CA3-MF) Synapses
From: Chronic early life lead (Pb2+) exposure alters presynaptic vesicle pools in hippocampal synapses
|  | CA3 – Mossy Fiber Terminals | Dentate Gyrus – Perforant Pathway Terminals | ||||
|---|---|---|---|---|---|---|
|  | Control (Mean ± SEM) | Pb2+ (Mean ± SEM) | P value | Control (Mean ± SEM) | Pb2+ (Mean ± SEM) | P value |
I. Raw Vesicle Counts | ||||||
 A) Rapidly Releasable/Docked Vesicle Pool | 8.527 ± 0.8456 | 6.018 ± 0.001 | 0.0236 | 7.013 ± 1.003 | 5.232 ± 0.9928 | 0.0989 |
 B) Recycling Vesicle Pool | 35.19 ± 7.804 | 29.26 ± 7.381 | 0.0187 | 31.48 ± 9.315 | 26.09 ± 5.835 | 0.1814 |
 C) Resting Vesicle Pool | 43.64 ± 5.570 | 51.80 ± 7.427 | 0.2271 | 51.66 ± 8.374 | 59.80 ± 11.44 | 0.2586 |
 D) Total Number of Vesicles | 87.36 ± 10.61 | 87.17 ± 1.761 | 0.4936 | 90.07 ± 9.363 | 91.12 ± 10.46 | 0.4603 |
II. Vesicular Diameters | ||||||
 A) Rapidly Releasable/Docked Diameter | 20.67 ± 1.644 | 21.20 ± 1.137 | 0.3995 | 20.82 ± 1.521 | 23.27 ± 2.12 | 0.3894 |
 B) Recycling Vesicle Diameter | 22.09 ± 0.8383 | 22.77 ± 0.6942 | 0.2799 | 22.10 ± 0.8234 | 24.41 ± 2.072 | 0.1695 |
 C) Resting Vesicle Diameter | 24.83 ± 1.746 | 24.70 ± 1.322 | 0.4764 | 24.69 ± 2.481 | 26.28 ± 2.506 | 0.3343 |
 D) Average Diameter of all vesicles | 23.77 ± 1.406 | 23.84 ± 0.8775 | 0.4843 | 23.72 ± 1.565 | 25.64 ± 2.094 | 0.2449 |
III. Length Measurements | ||||||
 A) PSD length | 309.1 ± 13.48 | 259.7 ± 18.74 | 0.0382 | 269.3 ± 9.680 | 266.6 ± 15.31 | 0.4432 |
 B) PAZ length | 311.6 ± 11.15 | 281.2 ± 19.53 | 0.0899 | 265.6 ± 14.45 | 308.2 ± 26.90 | 0.1553 |
IV. Nearest Neighbor Distance (Clustering) | ||||||
 A) Rapidly Releasable/Docked + Recycling (0–200 nm) | 40.19 ± 1.398 | 45.56 ± 1.498 | 0.0062 | 41.75 ± 0.7873 | 48.57 ± 1.115 | 0.0001 |
 B) Resting (201–500 nm) | 42.04 ± 1.834 | 48.47 ± 1.157 | 0.0020 | 45.35 ± 0.7979 | 51.19 ± 0.7168 | 0.0001 |
V. Mitochondrial Measurements | ||||||
 A) Terminals with mitochondria | 15.40 ± 2.227 | 17.60 ± 1.806 | 0.2340 | 27.00 ± 3.050 | 22.60 ± 3.280 | 0.1793 |
 B) Terminals with multiple mitochondria | 2.00 ± 0.5477 | 1.250 ± 0.2500 | 0.1316 | 4.200 ± 0.9695 | 2.600 ± 0.5099 | 0.0970 |
 C) Total number of mitochondria | 48.60 ± 6.577 | 54.20 ± 7.690 | 0.5972 | 69.60 ± 0.4964 | 62.80 ± 0.2518 | 0.1375 |
 D) Diameter of mitochondria | 231.6 ± 16.18 | 224.4 ± 15.53 | 0.7566 | 221.8 ± 0.8072 | 226.5 ± 8.529 | 0.3500 |
 E) Large mitochondria (300 nm or greater) | 4.800 ± 1.594 | 6.800 ± 0.9695 | 0.1624 | 8.00 ± 2.345 | 7.200 ± 1.530 | 0.3924 |