Fig. 3From: High content imaging quantification of multiple in vitro human neurogenesis events after neurotoxin exposureEffects of long term Bis1 exposure on neural differentiation and neurite extension. Cells were cultured on 96 well plates and continuously exposed to a range of doses of Bis1 through DIV 14. Cultures were then analyzed by immunocytochemistry staining for HuC/D along with βIII-tubulin followed by automated image acquisition and processing. a: Quantification of total cell count (total live cells using Hoechst 33342 positive nuclei). b: Quantification of neuron density (HuC/D+). Neuron density was measured as an indicator of cell health. c, d, e: Neurites per neuron, neurite length per neuron, branch points per neuron were also measured. All data are presented as % change from untreated control wells. Total live cell count, neuron density and neurite outgrowth data are from 2 separate experiments using independent cultures (n = 11 wells total). *Concentration is significant different from control group (P < 0.05, one-way ANOVA)Back to article page