Fig. 3

The involvement of Atg16L1 in the negative regulation of RSV on TNF-α induced ICAM-1 mRNA expression in HCAECs. HCAECs were either untreated (CON) or treated by TNF-α 10 ng/ml for 2 h in the presence or absence of RSV 10 μM (RSV) or Atg16L1 siRNA (siAtg16L1). Total RNA was prepared after infection and analyzed by real-time quantitative PCR to estimate amounts of ICAM-1 transcript. The amounts of ICAM-1 mRNA produced and normalized to the corresponding amount of GAPDH transcript were shown as the fold increase over untreated control cells. Results are represented as means ± S.E.M. for at least three determinations from independent experiments. (*p < 0.05 indicates significance, compared to TNF-α stimulation only)