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Table 1 Number of revertant colonies after POM93 exposure in absence and presence of S9 mix

From: Genotoxicity and acute and subchronic toxicity studies of a bioactive polyoxometalate in Wistar rats

Concentration (μg/plate)

Type of bacteria (mean ± SD)

TA97

TA98

TA100

TA102

A. Without S9 mix

    

5000

139.7 ± 14.5

33.7 ± 5.8

162.7 ± 14.0

264.0 ± 24.4

1000

158.7 ± 29.2

36.3 ± 3.7

187.7 ± 16.6

280.0 ± 23.5

200

168.7 ± 12.0

35.7 ± 6.3

162.0 ± 9.5

290.3 ± 16.0

40

167.7 ± 9.0

41.0 ± 3.6

179.7 ± 4.5

273.7 ± 36.6

8

165.3 ± 21.2

32.7 ± 6.6

160.0 ± 22.1

297.7 ± 10.0

0

150.7 ± 5.0

33.0 ± 3.6

171.3 ± 6.8

288.7 ± 10.0

Positive control

1748.7 ± 39.5*a

2118.7 ± 94.0*b

1760.0 ± 59.7*c

1817.7 ± 24.1*d

B. With S9 mix

    

5000

171.3 ± 11.7

40.7 ± 5.0

167.0 ± 38.0

284.3 ± 13.0

1000

163.3 ± 14.3

41.7 ± 2.5

155.0 ± 32.5

272.0 ± 34.0

200

184.0 ± 16.0

38.3 ± 4.0

134.3 ± 22.8

260.3 ± 15.6

40

192.7 ± 15.8

33.7 ± 3.5

160.3 ± 17.2

268.3 ± 39.8

8

175.7 ± 22.0

33.7 ± 5.0

154.3 ± 29.1

297.3 ± 9.6

0

173.0 ± 22.6

32.0 ± 5.2

173.0 ± 10.8

274.3 ± 17.4

Positive control

1690.7 ± 53.4*e

1687.7 ± 46.7*f

1184.7 ± 52.0*g

1107.3 ± 81.0*h

  1. No significant changes of revertants were observed at any concentrations of POM93 treatments in four tester strains. Statistical analysis: *P < 0.05 indicates a statistical difference with the control group by one-way ANOVA when the number of revertants was twice than control at TA97, TA98, TA100, and TA102
  2. Positive control in w/o S9 condition: a2,4,7-Trinitrofluorenone (TNF) 0.2 μg/plate; b2,4,7-Trinitrofluorenone (TNF) 0.2 μg/plate; cMethyl Methanesulfonate (MMS) 1.0 μL/plate; dMethyl Methanesulfonate (MMS) 1.0 μL/plate; e2-(2-furyl)-3-(5-nitro-2-fury)acrylamide (2-AF) 0.01 μg/plate; f2-(2-furyl)-3-(5-nitro-2-fury)acrylamide (2-AF) 0.01 μg/plate; g2-(2-furyl)-3-(5-nitro-2-fury)acrylamide (2-AF) 0.01 μg/plate; h2-aminoanthracene (2AA) 10.0 μg/plate