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Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: Knockdown of subunit 3 of the COP9 signalosome inhibits C2C12 myoblast differentiation via NF-KappaB signaling pathway

Fig. 1

Down regulation of CSN3 in C2C12 cell lines. a Representation of the CSN3 gene with arrows indicating the shRNAs target regions. b Low passage C2C12 were infected with lentiviral vectors expressing shCSN3-Med, shCSN3-Low or non-target shRNA (shNT). Stable cells lines were selected with puromycin (1.5 μg/ml). Total protein (20 μg) was analyzed by immunoblots using CSN3 and GAPDH (internal control) antibodies. A representative blot is shown from samples separated on a single gel. c CSN3 expression was quantified and normalized to GAPDH. Data represent means ± SEM for 7–8 independent samples. Data were analyzed by one-way ANOVA, ***P <0.001 compared to shNT-control

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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