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Table 1 Doses of methylxanthines injected per embryo in nanogram

From: Methylxanthines induce structural and functional alterations of the cardiac system in zebrafish embryos

Methylxanthines Injected dose (ng/ embryo)
Aminophylline 0.5 1.25 2.5* 3.75 5
Caffeine 0.25 0.5 0.75* 1.5 2.5
Diprophylline 1 2.5 5* 10 20
Doxofylline 1 2 3* 5 7.5
Etophylline 0.5 1 2* 3 5
IBMX 0.15 0.25 0.5* 0.75 1
Pentoxifylline 0.15 0.5 1* 2 3
Theophylline 0.25 0.5 1* 1.5 2.5
Dichloroaniline 0.015 0.05 0.1*   
Metoprolol 0.005 0.01 0.018* 0.037 0.075
  1. The doses labeled with asterisk (*) were selected for the experiments in the transgenic tg (Bmp:EGFP) embryos to study the long term effect of methylxanthines in heart rate. Dichloroaniline was used as positive control in evaluation of mortality and general morphology, and  metoprolol was used  as internal control in evaluation of heart rate