Fig. 5

Apoptosis assessment using Annexin V/7-AAD staining Fluorescent microscopic analysis of SHED cells stained with Annexin V/7-AAD. Yellow and red indicates early and late apoptotic cells, respectively. Shown are representative images of three independent experiments. SHED cells in direct contact with MTA for 2 days were positively stained by Annexin V. White bar, 20µm