Fig. 5

GPR120 is expressed in GH3 cells and did not influence GH3 cell viability. a: GPR120 transcription in GH3 cells was shown by RT-PCR. Lanes 1-3 were cDNA marker, GPR120 amplification products from GH3 cells and negative control, respectively. The size of PCR product is 340 bp. b: The protein expression of GPR120 in GH3 cells was shown by western blot. Lane 1 was the immunostaining of GPR120 and lane 2 was the protein marker. c: MTT assay of GH3 cell viability in response to GPR120 agonists and grifolic acid. ** means P < 0.01 vs control, n = 12