Fig. 2

Evaluation of cell death mechanism by quantifying the percentage of Hepa1c1c7 cells undergoing apoptosis, late apoptosis and necrosis, by flow cytometry using Annexin V / PI kit. The Hepa1c1c7 cells were treated with different concentrations of PHO-S, DODAC/PHO-S and DODAC, for a period of 12 h. a representative dot plots of fluorescence levels obtained by staining the cells for Annexin V/PI kit. b The bar chart shows the correlation of the effect expressed as a mean ± SD of three independent experiments. Level of statistical significance * P < 0.05, ** p < 0.01 and *** p < 0.001