Fig. 3

SBF-1 induced apoptosis and inhibited T cell cytokines production of activated T lymphocytes. a T cells were isolated from lymph node of BALB/c mice and then activated with 5 μg/ml Con A for 24 h at 37 °C. After being incubated with SBF-1 for 24 h, cells were collected and stained with Annexin V/PI to analyze apoptosis by flow cytometry. Data shown here are one of three different experiments with similar results. b–c T cells were isolated from lymph node of BALB/c mice and then incubated with SBF-1 in the presence of 5 μg/ml Con A for indicated time at 37 °C. b T cells were incubated for 24 h, and then they were collected. The mRNA expression of IL-2 and IFN-γ were determined by RT-PCR. Data shown here are one of three different experiments with similar results. c T cells were incubated for 72 h, and then cell culture supernatants were harvested. The levels of IL-2 and IFN-γ were measured by ELISA assay. Data are mean ± SEM of three independent experiments. P values were determined by one-way ANOVA with Tukey’s correction. *P < 0.05, **P < 0.01