Fig. 1

Conditioned medium from adipocytes reduces the lapatinib-induced cell cycle blockade in tumor cells. A) Lapatinib-induced cell cycle blockade was investigated on SKBR-3 cells incubated in control medium (a) or in adipocyte-conditioned medium (#3T3-CM) (b). Cells were exposed for 24 h to lapatinib before staining by propidium iodide and FACS analyses were performed to evaluate the percentage of cells in the different cell cycle phases. n ≥ 3. B) The expression of genes involved in the cell cycle progression was measured on tumor cells after exposure to lapatinib in presence (+) or not (−) of #3T3-CM. Cells were exposed for 12 h to lapatinib before RNA extraction and mRNA measurement by SYBR Green device. Fold changes were normalized for the cells exposed to lapatinib on the cells in the same condition not exposed to lapatinib. n ≥ 3