Fig. 2

Adipocyte-conditioned medium induces a tumor cell resistance to various tyrosine kinase inhibitors in Her2 positive cell lines. A) BT-474, SKBR3, MDA-MB-453, MDA-MB-361, MDA-MB-231 and MCF-7 tumor cell lines were incubated 24 h in adipocyte-conditioned medium (#3T3-CM) or in control medium then we added 0,1 μM of lapatinib and we measured the mitochondrial metabolic activity by MTT that we correlated with the percentage of viable cells. n ≥ 3. P values were calculated by comparing for each cell line the percentage of viable cells in presence of #3T3-CM to the percentage of viable cells in control medium after exposure to lapatinib. B) Under the same conditions of incubation as in A) BT-474 cells were exposed to tyrosine kinase inhibitors lapatinib, ibrutinib, afatinib and AZD4547. n ≥ 3. The IC50 values for each therapeutic agent were measured and we calculated the ratio and evaluated the p values of the value in presence of #3T3-CM to the control media condition. *p < 0,05 **p < 0,01