Fig. 5

Thermolabile soluble factor(s) are responsible for adipocyte-conditioned medium-induced resistance of breast cancer cells to lapatinib. A) adipocyte-conditioned medium (#3T3-CM) were heated at 95 °C in order to denature the protein and delipidated BSA was added to the CM prior to exposure of tumor cells and to lapatinib. n ≥ 3. B) #3T3-CM were centrifuged to separate the microvesicles then ultracentrifuged to precipitate the exosomes and to isolate soluble factors. n ≥ 3. P values were calculated by comparing the conditions to the control medium. * p < 0,05