Fig. 3
From: Two novel anticancer compounds with minimum cardiotoxic property
Annexin-V/PI flow cytometric analysis to quantify apoptosis induction in H9c2 cells. Dot plot of (a) H9c2 cells alone and treated with compound A at (b) 10 μM, (c) 50 μM and compound B at (d) 10 μM, (e) 50 μM for 24 h; (f) H9c2 cells alone and treated with compounds (g) A and (h) B at 10 μM for 48 h The results shown are representatives of three independent experiments. Quadrant 3, living cells An−/PI−; Quadrant 4, early apoptotic cells An+/PI−; Quadrant 2, late apoptotic cells An+/PI+; Quadrant 1, necrotic cells An−/ PI−