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Fig. 2 | BMC Pharmacology and Toxicology

Fig. 2

From: Gambogic acid protects LPS-induced apoptosis and inflammation in a cell model of neonatal pneumonia through the regulation of TrkA/Akt signaling pathway

Fig. 2

The effect of GA on LPS-induced WI-38 apoptosis and inflammation.aWI-38 cells were cultured in vitro (Ctrl), or treated with 10mg/ml LPS for 24h (LPS), or pre-incubated with 50 nM GA for 24h prior to LPS treatment (LPS + GA). A TUNEL assay was applied to identify apoptotic cells with TUNEL (Red) immunoreaction. During the meantime, DAPI (Blue) immunoreaction was applied to identify WI-38 cell nuclei. bFor images acquired in TUNEL assay, the percentages of apoptotic WI-38 cells were compared among Ctrl, LPS and LPS + GA conditions (* P < 0.05). cFor WI-38 cells under Ctrl, LPS and LPS + GA conditions, western blot analysis were conducted to compare IL-6 and MCP-1 protein expressions. dFor western blot data in (c), relative band intensities of IL-6 and MCP-1 were compared between LPS and LPS + GA conditions (* P < 0.05)

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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