Fig. 1

Sunitinib-induced disruption of cardiac sarcomeres in iPSC-CMs (A) Typical immunostaining results of iPSC-CMs following exposure to DMSO (control) or 3 μM sunitinib for 72 h. Nuclei are shown in blue, while sarcomeres are shown in green (60×; scale bar: 50 μm). (B) Quantitative analysis of sarcomere morphology via high-content image analysis. Data represent the percent change in the HSI of iPSC-CMs relative to the control and are expressed as mean ratios ± SD. Approximately 200 cells were analyzed in each well, one well was used for each data point, and five or six wells were used as the data for one group (n = 5–6 wells/group). **: p < 0.005, ***: p < 0.0005 versus control group using Shirley-Williams’ multiple comparison test