Fig. 6

Narciclasine mediates autophagy-dependent apoptosis of gastric cancer cells by inactivation of the Akt/mTOR pathway. A-C, Western blotting was used to detect the effect of narciclasine on total Akt and mTOR, p-Akt and p-mTOR of gastric cancer cells. D-I, Western blotting was employed to detect the effects of narciclasine combined with the Akt agonist insulin (200 nmol/L) and Akt inhibitor AktiVIII (10 μM) on Akt, mTOR, p-Akt, p-mTOR, and LC3-II protein after treatment of gastric cancer cells for 24 h. J-L, The apoptosis rate of gastric cancer cells treated with narciclasine and Akt agonist insulin (200 nmol/L) for 24 h was detected by flow cytometry. M-N, MMT assay was used to detect the 24-h cell viability of gastric cancer cells treated with narciclasine combined with the Akt agonist insulin (200 nmol/L). Data are shown as mean ± SD. NCS: narciclasine.ns: no significance