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Table 6 Lethality and signs of toxicity of mice administrated with berberine hydrochloride by iUDP

From: Improved up-and-down procedure for acute toxicity measurement with reliable LD50 verified by typical toxic alkaloids and modified Karber method

Seq. Dosage (mg/kg) Δm (g) Short-term outcome Symptoms Pathology
1 790 1.1 O Reduced activity, recovered after 2 h No visible alterations were found in organs and tissues
2 2500 1.5 O Reduced activity, recovered after 4.5 h No visible alterations were found in organs and tissues
3 5000 1.4 X Reduced activity, weakness, dead after 10 h Liver was congested
4 2500 0.9 O Reduced activity, recovered after 4.5 h No visible alterations were found in organs and tissues
5 5000 1.1 X Reduced activity, weakness, dead after 8 h Liver was congested
6 2500 1.7 X Reduced activity, dead after 16 h No visible alterations were found in organs and tissues
7 790 1.4 O Reduced activity, recovered after 1 h No visible alterations were found in organs and tissues
8 2500 1.1 O Reduced activity, recovered after 4 h No visible alterations were found in organs and tissues
9 5000 1.0 X Reduced activity, weakness, and dead after 18 h Liver was congested
Stop criteria met: 3 reversals in 5 tests
  1. Note: The sequence of outcomes: O for alive and X for dead