Fig. 3

Effect of Mito-DHA₅ on cell apoptosis and MMP in T24 cells. A T24 cells were treated with Mito-DHA₅ (0, 3, 10, 30 µM) for 48 h and then assayed by flow cytometry analysis with Annexin V-FITC staining. B Quantification of apoptotic cells. C Treat T24 cells with 30 µM of Mito-DHA₅ and DHA for 48 h, respectively, stained with Hochest 33,258, and viewed by fluorescence microscopy. Representative images were shown. Scale bar was 100 μm. D T24 cells were treated with Mito-DHA₅ (0, 3, 10, 30 µM) or DHA (30 µM) for 48 h and then viewed by fluorescence microscopy after JC-1 staining. Increased green fluorescence represents a decrease in MMP. Scale bar was 200 μm. E The percentage of green fluorescence and red fluorescence. Results are the mean±SD of three independent experiments. *P<0.5, **P<0.01, ***P<0.001, **** P<0.0001