Fig. 3

Cordycepin suppresses the relative MYC expression in colon cancer cells. A The relative MYC expression was measured via qRT-PCR in HCT116 cells. B Western blotting assay for the levels of MYC in HCT116 cells and Caco-2 cells with or without cordycepin treatment for 72 h. C Western blotting assay for the levels of MYC in HCT116 cells and Caco-2 cells with or without MYC over-expression. D HCT116 cells and Caco-2 cells were incubated with or without cordycepin, and cell proliferation was determined using cell number assay. E HCT116 and Caco-2 cells were incubated with or without cordycepin for 72 h, and the cell proliferation was determined using MTT assay. F HCT116 cells and Caco-2 cells were subjected to colony formation assay with or without cordycepin for two weeks. Data were mean ± S.D. of three independent experiments, and each measured in triplicate (**p < 0.01)