Fig. 4

Lentinan downregulated arsenic-induced inflammatory signals and upregulated anti-oxidative signals in liver by western-blotting analysis. A Characteristics of OX40, IL-17A, and NLRP3 inflammatory signaling after SA exposure or Lentinan intervention. SA induced a significant upregulations of OX40, IL-17A, and NLRP3, which were antagonized significantly after the intervention with Lentinan (P < 0.05); B Characteristics of Nrf2, NQO1 after SA exposure or Lentinan intervention. SA induced the upregulations of Nrf2 and NQO1, while the intervention with Lentinan showed the further upregulations of Nrf2 and NQO1 compared to SA treatment group (P < 0.05). Data were expressed as mean ± SD; n = 5. The levels of proteins expression were evaluated by the measurement of relative IA levels, quantified by the ratio of detected protein vs. internal control (β-actin) in each group (IA/IA). The significant difference between groups was obtained by independent sample’s t-test followed ANOVA. ^*P < 0.05 indicates a significant difference compared with control group; ^#P < 0.05 indicates a significant difference compared with SA treatment group. Abbreviations: NLRP3, NOD-like receptor family pyrin domain-containing 3; Nrf2, NF-E2 p45-related factor 2; NQO1, NAD(P)H quinone dehydrogenase 1; IA, integrated absorbance