Fig. 5From: Analysis of lung cancer-related genetic changes in long-term and low-dose polyhexamethylene guanidine phosphate (PHMG-p) treated human pulmonary alveolar epithelial cellsRT-qPCR validation for upregulated protein coding genes in the long-term treatment group. To validate the upregulated candidate genes on total RNA sequencing, the expression of MX1, KRT19, HMGA2, ISG15, IL33, MMP1, TRPA1, IFI6, PLAU, CDKN1A, PLAT, AK5 and NT5E was determined by RT-qPCR. All selected genes were verified to be increased in the long-term treatment group (within 35Â days from 27Â days). All experiments were performed 3 times with technical replicatesBack to article page