Fig. 2

Fasudil inhibited LPS-induced inflammatory factors expression in rat PMVECs. A and B Effect of LPS (10 μg/ml) on the mRNA expressions of IL-6, TNF-α and MCP-1 in rat PMVECs. Intensity of IL-6, TNF-α and MCP-1 was standardized to that of β-actin respectively. Values were presented as means ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01 versus the value of control group (LPS 0 h). C and D Fasudil inhibited LPS-induced mRNA expressions of IL-6 and MCP-1 in rat PMVECs. Quiescent PMVECs were incubated with vehicle or fasudil for 2 h, followed by 6 h stimulation with LPS (10 μg/ml) to detect the mRNA expression of MCP-1, and for 12 h stimulation to detect the mRNA expression of IL-6. Intensity of IL-6 and MCP-1 was standardized to that of β-actin. Values were presented as means ± SD of three independent experiments. ^##P < 0.01 vs control group; ^*P < 0.05, ^**P < 0.01 vs LPS-alone group. E and F Fasudil inhibited LPS-induced protein secretion of IL-6 and MCP-1 in the cultural medium of rat PMVECs. Quiescent PMVECs were incubated with vehicle or fasudil for 2 h, followed by stimulation with LPS (10 μg/ml) for indicated durations. The protein concentrations of IL-6 and MCP-1 in culture medium of rat PMVECs were detected respectively by ELISA. Values were presented as means ± SD. ^##P < 0.01 vs the control group (whithout LPS treatment) in each time point; ^*P < 0.05, ^**P < 0.01 versus the LPS-alone group in each time point. ^aP < 0.05, ^aaP < 0.01vs the group of LPS 0 h