Fig. 1
A The chemical structure of ethacrynic acid (EA; 1). B The inhibition of FXIIIa by EA (●) was measured spectro-fluorometrically through a bisubstrate, fluorescence-based trans-glutamination assay (λEx. = 360 nm and λEm. = 490 nm) at pH 7.4 and 37 °C. Solid lines represent sigmoidal fits to the data to obtain IC50, HS, YM, and YO using Eq. 1. See details in Methods part. Assay was repeated three times