Fig. 6

Differentiation of neuronal cells in cerebral organoids after resveratrol (RSV) treatment. A-C, at D20 in vitro culture, the cortical morphology of different groups under an inverted microscope, the NRs are thicker in the control group and the RSV-M, while the NRs in the D-gal are not observed. D-I, at D20 and D40, a large number of β-III-tublin-positive cells are distributed radially around the basal side of the lumen. A layer of collagen IV-positive basement membrane is wrapped around the NRs’ periphery, β-III-tublin-positive cells are decreased in the D-gal group compared with that in the control group, and β-III-tublin-positive cells are increased after RSV treated compared with that in the D-gal. A-C, scale bars = 200 μm; D-I, scale bars = 50 μm