Fig. 1

Groups receiving lactoferrin by enteral administration (E-LF) (A) or by perfusate (P-LF) (B). (A) Enteral administration groups. The E-LF 7.5 m, 15 m, 30 m, and 60 m groups received lactoferrin by gavage 7.5, 15, 30, and 60 min, respectively, before intraperitoneal pentobarbital injection. The control group received normal saline by gavage 30 min before intraperitoneal injection. All groups were perfused with KH buffer throughout. (B) Perfusate administration groups. The P-LF 15 and P-LF 100 groups received 15 mg/L and 100 mg/L lactoferrin, respectively, in KH buffer. The control group was perfused with KH buffer throughout. E-LF: enteral lactoferrin, KH: Krebs-Henseleit, p-Akt: phospho-protein kinase B, P-LF: perfusate lactoferrin