Fig. 3From: Celecoxib activates autophagy by inhibiting the mTOR signaling pathway and prevents apoptosis in nucleus pulposus cellsCXB enhanced autophagy in NPCs transfected with mRFP-GFP-LC3. A MOI titration assay for the optimal transfection. B Representative images and numbers of puncta of NPCs transfected with mRFP-GFP-LC3 for 6 h and replaced with fresh culture medium and subjected to IL-1β alone or with 2 µM or 10 µM CXB. Yellow puncta indicate autophagosome formation and were quantified (C). Each experiment was repeated three times. Scale bars for MOI titration, 200 μm. Scale Bars for autophagy, 30 μmBack to article page