Fig. 6

CXB has protective effects on IL-1β treated NPCs, and inhibition of autophagy leads to apoptosis. A Atg5 silencing and autophagy inhibition was evaluated by two different sequences of siRNA. B-E NPCs were treated with IL-1β, 10 µM CXB, or with inhibited autophagy. Pro-survival protein Bcl-2 and pro-apoptotic protein cleaved caspase-9, Bax were analyzed by Western blot. The grouping of blots were cropped from different parts of different blots. F, G For apoptosis analysis, NPCs were treated with IL-1β, 10 µM CXB, or inhibited with autophagy, then stained with YO-PRO-1/PI for FACS. Values in dot plots were represented in percentage. Data are represented as mean with SD of three repeats. *P ≤ 0.05, **P ≤ 0.01, *** P ≤ 0.001